Journal: Pharmaceutics

Article Title: Olaparib Conjugates with Selenopheno[3,2- c ]quinolinone Inhibit PARP1 and Reverse ABCB1-Related Multidrug Resistance

doi: 10.3390/pharmaceutics14122571

Figure Lengend Snippet: Cytotoxicity of 5a – c on the MES-SA/Dx-5 cell line.

Article Snippet: MES-SA (Human uterine sarcoma, ATCC ® CRL-1976TM) and H9C2 (rat cardiomyocytes, ATCC CRL-1446TM), MCF-7 (human adenocarcinoma, ATCC HTB-22TM), and HCC1937 (human breast carcinoma, ATCC CRL-2336TM) cell lines were obtained from American Type Culture Collection, and the MES-SA/Dx-5 (doxorubicin resistant human uterine sarcoma with high levels of MDR1 mRNA and P-glycoprotein, ECACC 95051031-1VL) cell line was obtained from the European Collection of Authenticated Cell Cultures.

Techniques:

Journal: Journal of Oncology

Article Title: GEMIN6 Overexpression Correlates with the Low Immune Cell Infiltration and Poor Prognosis in Lung Adenocarcinoma

doi: 10.1155/2022/1930604

Figure Lengend Snippet: GEMIN6 expression in LUAD and other types of human cancers from TCGA data. (a) GEMIN6 expression in different tumor types; (b) the expression of GEMIN6 in LUAD and its paired adjacent tissues; (c) the expression of GEMIN6 in LUAD and normal tissues; (d) the association of GEMIN6 expression and T stages in LUAD; (e) the association of GEMIN6 expression and pathologic stages in LUAD; (f) the association of GEMIN6 expression and N stages in LUAD; (g) relative expression of GEMIN6 in human bronchial epithelial cells (BEAS-2B) and LUAD cell lines (A549, H1299, SK-MES-1, PC-9, and NCI-H23); (h) receiver operating characteristic analysis (ROC) of GEMIN6 in LUAD.

Article Snippet: The A549, H1299, SK-MES-1, PC-9, and NCI-H23 LUAD cell lines and the normal human bronchial epithelial cell line BEAS-2B were purchased from the iCell Bioscience Inc. (China).

Techniques: Expressing

Journal: Oncotarget

Article Title: Dual-strand tumor-suppressor microRNA-145 ( miR-145-5p and miR-145-3p ) coordinately targeted MTDH in lung squamous cell carcinoma

doi: 10.18632/oncotarget.12290

Figure Lengend Snippet: A. Expression levels of miR-145-5p and miR-145-3p in lung SCC clinical specimens and cell lines (SK-MES-1 and EBC-1) were determined by qRT-PCR. Data were normalized to RNU48 expression. B. Correlation of the expression levels of miR-145-5p and miR-145-3p . C. Cell growth was determined by XTT assays 72 h after transfection with 10 nM miR-145-5p or miR-145-3p . * P < 0.05. D. Cell migration activity was determined by wound healing assays. * P < 0.001. E. Cell invasion activity was determined using Matrigel invasion assays. * P < 0.001.

Article Snippet: Two human lung SCC cell lines (SK-MES-1 and EBC-1) were obtained from Japanese Cancer Research Resources Bank (JCRB) and the American Type Culture Collection (Manassas, VA, USA), respectively.

Techniques: Expressing, Quantitative RT-PCR, Transfection, Migration, Activity Assay

Journal: Oncotarget

Article Title: Dual-strand tumor-suppressor microRNA-145 ( miR-145-5p and miR-145-3p ) coordinately targeted MTDH in lung squamous cell carcinoma

doi: 10.18632/oncotarget.12290

Figure Lengend Snippet: A. Flow chart illustrates the strategy for analysis of miR-145-5p and miR-145-3p target genes in lung SCC cells. B. Identification of target genes of miR-145-5p or miR-145-3p . Expression levels of 7 mRNAs ( MTDH , EPN3 , TPD52 , CYP27B1 , LMAN1 , STAT1 and TXNDC12 ) were evaluated by qRT-PCR in EBC-1 cells 72 h after transfection with miR-145-5p or miR-145-3p . GUSB was used as an internal control. * P < 0.0001.

Article Snippet: Two human lung SCC cell lines (SK-MES-1 and EBC-1) were obtained from Japanese Cancer Research Resources Bank (JCRB) and the American Type Culture Collection (Manassas, VA, USA), respectively.

Techniques: Expressing, Quantitative RT-PCR, Transfection, Control